“Candidatus Liberibacter asiaticus” Prophage Late Genes May Limit Host Range and Culturability
Identifieur interne : 000848 ( Main/Exploration ); précédent : 000847; suivant : 000849“Candidatus Liberibacter asiaticus” Prophage Late Genes May Limit Host Range and Culturability
Auteurs : Laura A. Fleites ; Mukesh Jain ; Shujian Zhang ; Dean W. GabrielSource :
- Applied and Environmental Microbiology [ 0099-2240 ] ; 2014.
Abstract
“
Url:
DOI: 10.1128/AEM.01958-14
PubMed: 25063651
PubMed Central: 4178692
Affiliations:
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Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">“<named-content content-type="genus-species">Candidatus</named-content>
Liberibacter asiaticus” Prophage Late Genes May Limit Host Range and Culturability</title>
<author><name sortKey="Fleites, Laura A" sort="Fleites, Laura A" uniqKey="Fleites L" first="Laura A." last="Fleites">Laura A. Fleites</name>
</author>
<author><name sortKey="Jain, Mukesh" sort="Jain, Mukesh" uniqKey="Jain M" first="Mukesh" last="Jain">Mukesh Jain</name>
</author>
<author><name sortKey="Zhang, Shujian" sort="Zhang, Shujian" uniqKey="Zhang S" first="Shujian" last="Zhang">Shujian Zhang</name>
</author>
<author><name sortKey="Gabriel, Dean W" sort="Gabriel, Dean W" uniqKey="Gabriel D" first="Dean W." last="Gabriel">Dean W. Gabriel</name>
</author>
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Liberibacter asiaticus” Prophage Late Genes May Limit Host Range and Culturability</title>
<author><name sortKey="Fleites, Laura A" sort="Fleites, Laura A" uniqKey="Fleites L" first="Laura A." last="Fleites">Laura A. Fleites</name>
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<author><name sortKey="Jain, Mukesh" sort="Jain, Mukesh" uniqKey="Jain M" first="Mukesh" last="Jain">Mukesh Jain</name>
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<author><name sortKey="Zhang, Shujian" sort="Zhang, Shujian" uniqKey="Zhang S" first="Shujian" last="Zhang">Shujian Zhang</name>
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<author><name sortKey="Gabriel, Dean W" sort="Gabriel, Dean W" uniqKey="Gabriel D" first="Dean W." last="Gabriel">Dean W. Gabriel</name>
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<series><title level="j">Applied and Environmental Microbiology</title>
<idno type="ISSN">0099-2240</idno>
<idno type="eISSN">1098-5336</idno>
<imprint><date when="2014">2014</date>
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<front><div type="abstract" xml:lang="en"><p>“<named-content content-type="genus-species">Candidatus</named-content>
Liberibacter asiaticus” is an uncultured alphaproteobacterium that systemically colonizes its insect host both inter- and intracellularly and also causes a severe, crop-destroying disease of citrus called huanglongbing, or citrus “greening.” <italic>In planta</italic>
, “<italic>Ca</italic>
. Liberibacter asiaticus” is also systemic but phloem limited. “<italic>Ca</italic>
. Liberibacter asiaticus” strain UF506 carries two predicted prophages, SC1 and SC2. Bacteriophage particles have been observed in experimentally “<italic>Ca</italic>
. Liberibacter asiaticus”-infected periwinkle but not in any other host. Comparative gene expression analysis of predicted SC1 late genes showed a much higher level of late gene expression, including holin transcripts (SC1_gp110), in “<italic>Ca</italic>
. Liberibacter asiaticus”-infected periwinkle relative to “<italic>Ca</italic>
. Liberibacter asiaticus”-infected citrus. To functionally characterize predicted holin and endolysin activity, SC1_gp110 and two predicted endolysins, one within SC1 (SC1_gp035) and another well outside the predicted prophage region (CLIBASIA_04790), were cloned and expressed in <named-content content-type="genus-species">Escherichia coli</named-content>
. Both SC1 genes inhibited bacterial growth consistent with holin and endolysin function. The holin (SC1_gp110) promoter region was fused with a <italic>uidA</italic>
reporter on pUFR071, a wide bacterial host range (repW) replicon, and used to transform <named-content content-type="genus-species">Liberibacter crescens</named-content>
strain BT-1 by electroporation. BT-1 is the only liberibacter strain cultured to date and was used as a proxy for “<italic>Ca</italic>
. Liberibacter asiaticus.” pUFR071 was >95% stable without selection in BT-1 for over 20 generations. The reporter construct exhibited strong constitutive glucuronidase (GUS) activity in culture-grown BT-1 cells. However, GUS reporter activity in BT-1 was suppressed in a dose-dependent manner by crude aqueous extracts from psyllids. Taken together with plant expression data, these observations indicate that “<italic>Ca</italic>
. Liberibacter asiaticus” prophage activation may limit “<italic>Ca</italic>
. Liberibacter asiaticus” host range and culturability.</p>
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<tree><noCountry><name sortKey="Fleites, Laura A" sort="Fleites, Laura A" uniqKey="Fleites L" first="Laura A." last="Fleites">Laura A. Fleites</name>
<name sortKey="Gabriel, Dean W" sort="Gabriel, Dean W" uniqKey="Gabriel D" first="Dean W." last="Gabriel">Dean W. Gabriel</name>
<name sortKey="Jain, Mukesh" sort="Jain, Mukesh" uniqKey="Jain M" first="Mukesh" last="Jain">Mukesh Jain</name>
<name sortKey="Zhang, Shujian" sort="Zhang, Shujian" uniqKey="Zhang S" first="Shujian" last="Zhang">Shujian Zhang</name>
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